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DAF-2DA

Cat. No. M29138
DAF-2DA Structure
Synonym:

5,6-Diaminofluorescein diacetat

Size Price Availability Quantity
50ug USD 110  USD110 In stock
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Quality Control & Documentation
Biological Activity

DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.

Chemical Information
Molecular Weight 446.41
Formula C24H18N2O7
CAS Number 205391-02-2
Storage Please store the product under the recommended conditions in the Certificate of Analysis.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Neidiquele Maria Silveira, et al. Methods Mol Biol. Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins

[2] Aakanksha Wany, et al. Methods Mol Biol. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence

[3] Luis A J Mur, et al. Plant Sci. Methods of nitric oxide detection in plants: a commentary

[4] Julia Schwendemann, et al. Gynecol Endocrinol. Nitric oxide detection by DAF (diaminofluorescein) fluorescence in human myometrial tissue

[5] Sanjoy Roychowdhury, et al. Glia. Oxidative stress in glial cultures: detection by DAF-2 fluorescence used as a tool to measure peroxynitrite rather than nitric oxide

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