D-Luciferin is a light-emitting compound, also a natural substrate of luciferases. D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO: ≥ 10 mg/mL (Need warming and ultrasonic)|
Hydrazide d-luciferin for in vitro selective detection and intratumoral imaging of Cu(2.).
Zheng Z, et al. Biosens Bioelectron. 2016 Sep 15;83:200-4. PMID: 27131992.
Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over D-luciferin.
Mofford DM, et al. J Am Chem Soc. 2014 Sep 24;136(38):13277-82. PMID: 25208457.
Timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growth using in vivo bioluminescence imaging.
Inoue Y, et al. Int J Biomed Imaging. 2010;2010:471408. PMID: 20671955.
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