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5(6)-Aminofluorescein

Cat. No. M4826
5(6)-Aminofluorescein Structure
Synonym:

5(6)-AFM; Fluoresceinamine mixed isomers

Size Price Availability Quantity
10mM*1mL in DMSO USD 65  USD65 In stock
100mg USD 60  USD60 In stock
500mg USD 100  USD100 In stock
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Quality Control & Documentation
Biological Activity

5(6)-Aminofluorescein is a high purity and quality chemical used as molecular probe and important as fluorescent agent.

Chemical Information
Molecular Weight 694.64
Formula C40H26N2O10
CAS Number 27599-63-9
Solubility (25°C) DMSO
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Hung-Yu Ko, et al. Determination of phenylenediamines in hair colors derivatizated with 5-(4, 6-dichlorotriazinyl)aminofluorescein via micellar electrokinetic chromatography

[2] Rodrigo P Feliciano, et al. Fluorescent labeling of cranberry proanthocyanidins with 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein (DTAF)

[3] Reinhard Ding, et al. Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgery

[4] A Ogamo, et al. Preparation and properties of fluorescent glycosaminoglycuronans labeled with 5-aminofluorescein

[5] R M McKinney, et al. Reaction paths to pure 5- and 6-aminofluorescein. Pitfalls encountered in conventional synthesis procedures

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Keywords: 5(6)-Aminofluorescein, 5(6)-AFM; Fluoresceinamine mixed isomers supplier, Fluorescent Dye, inhibitors, activators


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