Certificate of Analysis

 
Product Name: Recombinant Human HMGB1 Protein (E. coli)
Catalog Number: M55397
Physical and Chemical Properties
Form: Lyophilized powder
Solubility: Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage: Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Analytical Data
QC Standard: >95%, Endotoxin < 1 EU/µg
Product Information
Description: High mobility group protein B1 is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses.
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.