Certificate of Analysis

 

Product Name: Recombinant Human EGF Protein (HEK293, hFc)
Catalog Number: M22459

Physical and Chemical Properties
Solubility: Reconstitute the lyophilized powder in sterile water to prepare a stock solution of 250 μg/ml
Storage: Stored at ≤ -20°C, stable for one year after receipt.
Analytical Data
QC Standard: >90%, Endotoxin < 1 EU/µg
Product Information
Description: EGF is the founding member of the EGF-family of proteins. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells, the ED50 for this effect is typically 0.3-1.5 ng/mL.
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.