Certificate of Analysis

 

Product Name: Nile Blue
Catalog Number: M58558
CAS Number: 3625-57-8

Physical and Chemical Properties
Formula: C40H40N6O6S
Molecular Weight: 732.85
Form: Powder
Solubility: DMSO ≥ 50 mg/mL; Water ~1 mg/mL
Storage: RT, protect from light, dry, sealed
Chemical Structure: Nile Blue Structure
Analytical Data
QC Standard: Biological Stain, Dye content ≥75%
H-NMR: Consistent with structure
Product Information
Description: Nile Blue A is highly fluorescent, and photostable organic dye used in biology and histology. And Nile Blue A is for use in lipid histochemistry. Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red. Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.