Certificate of Analysis

 

Product Name: Hydroxyphenyl Fluorescein
Catalog Number: M58445
CAS Number: 359010-69-8

Physical and Chemical Properties
Formula: C26H16O6
Molecular Weight: 424.4
Form: Solid
Solubility: DMSO 65 mg/mL
Storage: -20°C, protect from light
Chemical Structure: Hydroxyphenyl Fluorescein Structure
Analytical Data
QC Standard: ≥99.0%
H-NMR: Consistent with structure
Product Information
Description: Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm.
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.