Certificate of Analysis

 

Product Name: HBC530
Catalog Number: M59029
CAS Number: 156840-13-0

Physical and Chemical Properties
Formula: C19H17N3O
Molecular Weight: 303.36
Form: Solid
Solubility: DMSO 65 mg/mL
Storage: 4°C, protect from light
Chemical Structure: HBC530 Structure
Analytical Data
QC Standard: ≥99.0%
H-NMR: Consistent with structure
Product Information
Description: HBC530 is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence (Kd of ~3.5 nM). HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC can be used in the live cell imaging of RNA (Em/Ex = 530/485 nm).
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.