Certificate of Analysis

 

Product Name: Fluo-5N,AM
Catalog Number: M21335

Physical and Chemical Properties
Molecular Weight: 1127.92
Solubility: Soluble in DMSO
Storage: Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Chemical Structure: Fluo-5N,AM Structure
Analytical Data
QC Standard:
Product Information
Description: Calcium fluorescent probe Fluo-5N,AM is a calcium fluorescent probe, Fluo-5N is an analog of Fluo-4 with a low calcium binding affinity (Kd = ~90 uM), making it suitable for detecting intracellular calcium levels in the range of 1 μM to 1 mM, which would saturate the Fluo-4 response. The optimal excitation/emission wavelength is 490 nm/525 nm.
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.