Certificate of Analysis
| Product Name: | Ethidium bromide |
| Catalog Number: | M40780 |
| CAS Number: | 1239-45-8 |
Physical and Chemical Properties
| Formula: | C21H20BrN3 |
| Molecular Weight: | 394.32 |
| Form: | Solid |
| Solubility: | H2O ≥ 100 mg/mL; DMSO 25 mg/mL |
| Storage: | 4°C, protect from light, dry, sealed |
| Chemical Structure: |  |
Analytical Data
| QC Standard: | >99.0% |
| H-NMR: | Consistent with structure |
Product Information
| Description: | Ethidium bromide is an embedded dye used for fluorescent labeling (nucleic acid staining) in molecular biology laboratory science, such as agarose gel electrophoresis. Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| Stability and Solubility Advice: | Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details. |