Certificate of Analysis
| Product Name: | 20α-Hydroxycholesterol |
| Catalog Number: | M58641 |
| CAS Number: | 516-72-3 |
Physical and Chemical Properties
| Formula: | C27H46O2 |
| Molecular Weight: | 402.65 |
| Form: | Solid |
| Solubility: | DMSO 9 mg/mL |
| Storage: |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Chemical Structure: |  |
Analytical Data
| QC Standard: | ≥99.0% |
| H-NMR: | Consistent with structure |
Product Information
| Description: | 20α-Hydroxycholesterol is an allosteric activator that selectively targets the Smoothened (Smo) of the Hedgehog pathway with an EC50 of ~30 μM (Hedgehog). 20α-Hydroxycholesterol binds to the extracellular cysteine-rich domain (CRD) of Smo in a stereoselective manner, activating downstream Gli transcription factors. 20α-Hydroxycholesterol enhances osteogenic differentiation of bone marrow stromal cells. |
| Stability and Solubility Advice: | Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details. |