Certificate of Analysis
| Product Name: | Hoechst 33258 |
| Catalog Number: | M11514 |
| CAS Number: | 23491-44-3 |
Physical and Chemical Properties
| Formula: | C25H24N6O |
| Molecular Weight: | 424.50 |
| Solubility: | DMSO ≥ 20 mg/mL |
| Storage: | -20°C, protect from light, dry, sealed |
| Chemical Structure: |  |
Analytical Data
| QC Standard: | >99% |
| H-NMR: | Consistent with structure |
Product Information
| Description: | Hoechst 33258 (dibiphenimide) can bind dsDNA, with a maximum excitation/emission wavelength of 352 nm/461 nm (after binding with DNA), giving blue fluorescence, which is often used to observe the nucleus and nucleic acid. Store at room temperature away from light. |
| Stability and Solubility Advice: | Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details. |