Free shipping on all orders over $ 500

ZM 336372

Cat. No. M1818
ZM 336372 Structure


Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mg USD 105 In stock
50mg USD 360 In stock
100mg USD 480 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

ZM 336372 is a potent, selective ATP-competitive inhibitor of c-Raf in vitro (IC50 = 70 nM for inhibition of human c-Raf). The Ras/Raf-1 signalling pathway is a well-characterized system that links receptor tyrosine kinase (RTK) activation with changes in gene expression and cell behavior. Raf-1 is a serine/threonine protein kinase that phosphorylates and activates MAPK-kinase (MEK) in response to activation by Ras. ZM 336372 also inhibits SAPK2/p38 (IC50 = 2 μM). Selective over 17 other protein kinases including PKA, PKC, p42 MAPK, CDK1 and SAPK1/JNK (up to 50 μM). ZM 336372 causes growth inhibition, suppression of hormone secretion, and up-regulation of cell cycle inhibitors in a human hepatocellular carcinoma cell line.

Customer Product Validations & Biological Datas
Source J Surg Res (2010). Figure 1. ZM 336372
Method MTT assay
Cell Lines pancreatic adenocarcinoma cells
Concentrations 100 μM
Incubation Time 8 d
Results Here we utilize the MTT assay to determine if there is a difference in cellular proliferation in response to treatment with ZM336372 in pancreatic adenocarcinoma cells
Cell Experiment
Cell lines H727 and BON cells
Preparation method Cell Proliferation Assay
Proliferation of H727 and BON cells after treatment with ZM336372 was measured using a 3,4-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Sigma). Cells were trypsinized and plated in triplicate to 24-well plates and allowed to adhere overnight. Then, cells were treated with either 100 μmol/L ZM336372 or DMSO (2%) and incubated. Media were changed every 2 days with new treatment. At each time point, cell growth rates were analyzed after the addition of 3,4-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reagent to the cultured cells following manufacturer's instructions. Absorbance was determined using spectrophotometer at a wavelength of 540 nm.
Concentrations 100 μmol/L
Incubation time 10 days
Animal Experiment
Animal models
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 389.45
Formula C23H23N3O3
CAS Number 208260-29-1
Purity >99%
Solubility DMSO ≥78 mg/mL
Storage at -20°C

ZM336372, a Raf-1 activator, causes suppression of proliferation in a human hepatocellular carcinoma cell line.
Deming D, et al. J Gastrointest Surg. 2008 May;12(5):852-7. PMID: 18299943.

ZM336372, a Raf-1 activator, suppresses growth and neuroendocrine hormone levels in carcinoid tumor cells.
Van Gompel JJ, et al. Mol Cancer Ther. 2005 Jun;4(6):910-7. PMID: 15956248.

Paradoxical activation of Raf by a novel Raf inhibitor.
Hall-Jackson CA, et al. Chem Biol. 1999 Aug;6(8):559-68. PMID: 10421767.

Related Raf Products

PLX7904, also known as PB04, is a potent and selective paradox-breaker RAF inhibitor.


PLX8394 is an orally bioavailable, potent and selective Raf inhibitor with IC50 of 5 nM.


B-Raf-IN-1 (MDK36057) is a potent b-Raf inhibitor with IC50 of 24 nM.


RAF709 is a novel Raf kinase inhibitor with IC50 values of 0.4 and 1.5 nM for c-Raf and b-Raf, respectively.


L-779450 is a potent, ATP-competitive Raf kinase inhibitor (IC50 =10 nM) that displays > 7, > 30 and > 70-fold selectivity over p38α, GSK3β and Lck respectively.

Abmole Inhibitor Catalog 2017

Keywords: ZM 336372, ZM336372 supplier, Raf, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.