XTT sodium salt is a tetrazolium salt, which forms water-soluble formazan on bioreduction. XTT sodium salt is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.
| Molecular Weight | 673.52 |
| Formula | C22H16N7NaO13S2 |
| CAS Number | 111072-31-2 |
| Solubility (25°C) | DMSO 3 mg/mL Warmed Water 2 mg/mL |
| Storage | 2-8°C, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Cell Analysis Products |
|---|
| Antifade Mounting Medium with DAPI
AbMole Antifade Mounting Medium with DAPI is a ready-to-use solution that can not only slow down the quenching of fluorescent dyes, but also complete the DAPI staining of cell nuclei while mounting the slides. |
| Antifade Mounting Medium
AbMole Antifade Mounting Medium is a sealing reagent used to slow the fluorescence quenching. It can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect. |
| WST-1
WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm. |
| MTT
MTT is a reagent used in the measurement of cell proliferation. |
| Cell Counting Kit-8
Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.
