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WZ4003

Cat. No. M3132
WZ4003 Structure
Size Price Availability Quantity
50mg USD 460 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

WZ4003 is a highly specific NUAK kinase inhibitor with IC50 of 20 nM and 100 nM for NUAK1 and NUAK2, respectively, without significant inhibition on 139 other kinases.

Protocol
Cell Experiment
Cell lines U2OS cells, and MEFs
Preparation method Following the manufacturer’s protocol ,to carry out cell proliferation assays colorimetrically in 96-well plates using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit . Initially, 2000 cells per well are seeded for U2OS cells and 3000 cells per well are seeded for MEFs. The proliferation assays are carried out over 5 days in the presence or absence of 10 μM WZ4003.
Concentrations ~10 μM
Incubation time 5 days
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 496.99
Formula C25H29ClN6O3
CAS Number 1214265-58-3
Purity >99%
Solubility DMSO
Storage at -20°C
Customer Product Validations & Biological Datas
Source APMIS.m (2017). Figure 7. WZ4003 (Abmole Bioscience Inc. Houston, TX, USA)
Method Western blot
Cell Lines HeLa cells
Concentrations
Incubation Time
Results Particularly, pifithrin-μ and WZ4003 dramatically decreased 10-G-induced activation of the p53 and p-AMPK protein, respectively.
Rating
Product Citations
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: WZ4003 supplier, AMPK, inhibitors

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