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Topotecan hydrochloride

Cat. No. M2144

Topotecan hydrochloride Structure

Synonym: Hycamtin, NSC 609669

Size Price Availability Quantity
50mg USD 110 In stock
100mg USD 180 In stock
500mg USD 420 In stock
1g USD 585 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Topotecan hydrochloride (trade name Hycamtin) is a potent antineoplastic agent. It is a water soluble analog of the alkaloid camptothecin that potently inhibits DNA topoisomerase I. Topotecan hydrochloride induces rapid apoptotic death in human B-lineage acute lymphoblastic leukemia (ALL) cells with IC50 values of 33 nM and 0.28 μM, respectively. Topotecan improves survival in three mouse models of poor prognosis ALL and downregulates B-Myb and MycN expression in neuroblastoma cells. Topotecan demonstrates a broad spectrum of antitumor activity against a variety of other tumors including ovarian cancer, cervical cancer, neuroblastoma, brainstem glioma, and Ewing’s sarcoma.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 457.91
Formula C23H23N3O5.HCl
CAS Number 119413-54-6
Purity >99%
Solubility DMSO 90 mg/mL
Water 90 mg/mL
Storage at -20°C
Customer Product Validation
Source J Korean Med Sci (2016). Figure 5. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
Method CCK8 Assay and ABCG2 ATPase assay
Cell Lines RCSC cells
Concentrations 0~10 μM
Incubation Time 72 h
Results MiR-3163 plays important roles in multidrug resistance (Table 1, Fig. 5A). Results showed that when miR-3163 was overexpressed in RCSCs, the ATPase activity of ABCG2 was significantly downregulated compared to RCSCs with Scramble (Fig. 5B).
Rating
Product Citations
References

A chemical screen identifies the chemotherapeutic drug topotecan as a specific inhibitor of the B-MYB/MYCN axis in neuroblastoma.
Sottile F, et al. Oncotarget. 2012 May;3(5):535-45. PMID: 22619121.

Bioavailability and pharmacokinetics of oral topotecan: a new topoisomerase I inhibitor.
Schellens JH, et al. Br J Cancer. 1996 May;73(10):1268-71. PMID: 8630291.

In vitro and in vivo activity of topotecan against human B-lineage acute lymphoblastic leukemia cells.
Uckun FM, et al. Blood. 1995 May 15;85(10):2817-28. PMID: 7742543.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Topotecan hydrochloride, Hycamtin, NSC 609669 supplier, Topoisomerase, inhibitors

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