Recombinant human TNF-α protein (E. coli) is a homotrimer with a subunit molecular mass of 17.3 kDa. TNF-a plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections.
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 is < 30.0 pg/ml, corresponding to a specific activity of > 3.3 × 107 units/mg.
Accession: # P01375
Apparent molecular weight: ~17.3 kDa, SDS-PAGE under reduction conditions.
Lyophilized after extensive dialysis against PBS.
Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Elissavet Kemanetzoglou, et al. Curr Neurol Neurosci Rep. CNS Demyelination with TNF-α Blockers
[2] Helga Radner, et al. Wien Med Wochenschr. Anti-TNF in rheumatoid arthritis: an overview
[3] T Tzanavari, et al. Curr Dir Autoimmun. TNF-alpha and obesity
[4] Frances Balkwill. Cancer Metastasis Rev. TNF-alpha in promotion and progression of cancer
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