Tetramethylrhodamine methyl ester (TMRM) is a cell permeable, cationic fluorescent dye with a maximum excitation/emission wavelength of 550nm/576nm. It emits red fluorescence and can easily adsorb active mitochondria. Store in refrigerator (-5 to -30°C) away from light.
Molecular Weight | 401.48 |
Formula | C25H25N2O3+ |
CAS Number | 115532-49-5 |
Solubility (25°C) | N/A |
Storage | -20°C, protect from light, dry, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[5] Brad Chazotte. Cold Spring Harb Protoc. Labeling mitochondria with MitoTracker dyes
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