Free shipping on all orders over $ 500

Stenabolic (SR9009)

Cat. No. M5143
Stenabolic (SR9009) Structure
Synonym:

Stenabolic; REV-ERB Agonist II

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM*1mL in DMSO USD 65  USD65 In stock
5mg USD 60  USD60 In stock
10mg USD 95  USD95 In stock
50mg USD 265  USD265 In stock
100mg USD 395  USD395 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Stenabolic (SR9009) is an agonist of REV-ERB, with IC50 of 670 nM and 800 nM for REV-ERBα and REV-ERBβ respectively. Stenabolic (SR9009) inhibits the activity of the SCN clock, with reversible inhibition of circadian oscillations in SCN explants cultured from the Per2:luc reporter mouse. Stenabolic (SR9009) dose-dependently increases the REV-ERB-dependent repressor activity assessed in HEK293 cells expressing a chimeric Gal4 DNA Binding Domain (DBD) - REV-ERB ligand binding domain (LBD) α or β and a Gal4-responsive luciferase reporter. Stenabolic (SR9009) potently and efficaciously suppresses transcription in a cotransfection assay using full-length REV-ERBα along with a luciferase reporter driven by the Bmal1 promoter (SR9009 IC50=710 nM).

Customer Product Validations & Biological Datas
Source Cell Physiol Biochem (2018). Figure 4. SR9009
Method MTT assay
Cell Lines The human monocytic leukemia cell line
Concentrations 1, 5, 10, and 50 µM
Incubation Time 12 h
Results Expression of hMMP-9 mRNA also decreased when cells were treated with SR9009 alone, without Ang II stimulation (P < 0.05). The effect of SR9009 was further confirmed on mouse MMP-9 (mMMP-9) mRNA expression in RAW264.7 macrophages
Protocol (for reference only)
Cell Experiment
Cell lines L929 cell
Preparation method Cells were treated with 100 ng/ml IFNg (Millipore) and 10 ng/ml LPS(Millipore) for M1 polarization. M2 differentiation was induced by incubating macrophages with 15 ng/ml IL-4 (Millipore).
Concentrations 10 μM
Incubation time 24 h
Animal Experiment
Animal models homozygous LDL receptor deficient (ldlr−/−) male mice
Formulation PBS
Dosages 100 mg/kg
Administration
Chemical Information
Molecular Weight 437.94
Formula C20H24ClN3O4S
CAS Number 1379686-30-2
Solubility (25°C) DMSO ≥ 60 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Geldof L, et al. Int J Mol Sci. In Vitro Metabolic Studies of REV-ERB Agonists SR9009 and SR9011.

[2] Solt LA, et al. Nature. Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists.

Related REV-ERB Products
SR12418

SR12418 is a REV-ERB-specific synthetic ligand with IC50 values of 68 nM for REV-ERBα and 119 nM for REV-ERBβ. SR12418 can be used in studies related to experimental autoimmune encephalomyelitis (EAE) and colitis.

SR-29065

SR-29065 is a selective REV-ERBα agonist for autoimmune disease research.

STL1267

STL1267 is a potent REV-ERB agonist that crosses the blood-brain barrier with a Ki value of 0.16 µM for REV-ERBα. STL1267 showed no cytotoxicity and inhibited BMAL1 gene expression.

SR9011 hydrochloride

SR9011 hydrochloride is a REV-ERBα/β agonist with IC50s of 790 nM and 560 nM for REV-ERBα and REV-ERBβ, respectively.

SR9011

SR9011 is a REV-ERBα/β agonist with IC50s of 790 nM and 560 nM for REV-ERBα and REV-ERBβ, respectively.

  Catalog
Abmole Inhibitor Catalog




Keywords: Stenabolic (SR9009), Stenabolic; REV-ERB Agonist II supplier, REV-ERB, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.