Solamargine is a major steroidal alkaloid glycoside extracted from a traditional Chinese medicinal herb, Solanum nigrum L. (SNL); has been shown to inhibit growth and induce apoptosis of various cancer cells.
| Cell Experiment | |
|---|---|
| Cell lines | H1650, H1975, A549, PC9 and H1299 cells |
| Preparation method | Cell viability assay Cell viability was measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Briefly, lung cancer cells were harvested and seeded in a 96-well microtiter plate followed by treating with solamargine (6 μM) for up to 48 hrs. Afterwards, MTT solution (20 μl, 5 g/l) was added to each well, and cells were incubated at 37°C for an additional 4 hrs. Finally, the 200-μl solvent dimethyl sul-foxide was added to each well for 10 min. The ELISA reader (Perkin Elmer, Victor X5, USA) was used to detect the Absorbance at 490 nm. Cell viability (%) was calculated as follows: (absorbance of test sample/ absorbance of control) 100%. |
| Concentrations | 6 μM |
| Incubation time | 48 h |
| Animal Experiment | |
|---|---|
| Animal models | A549 cells xenograft in nude mice |
| Formulation | saline |
| Dosages | 4 and 8mg/kg for 30 days |
| Administration | oral gavage |
| Molecular Weight | 868.06 |
| Formula | C45H73NO15 |
| CAS Number | 20311-51-7 |
| Solubility (25°C) | 10 mM in DMSO |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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