SN-38 is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks.
BMC Biol. 2021 May 20;19(1):108.
Very long intergenic non-coding (vlinc) RNAs directly regulate multiple genes in cis and trans
SN-38 purchased from AbMole
Nat Commun. 2019 Dec 20;10(1):5799.
Novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells.
SN-38 purchased from AbMole
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Source | Customer experimental result (2018). |
| Method | Cell viability assay | |
| Cell Lines | MDA-MB-231 cells | |
| Concentrations | 32nM | |
| Incubation Time | 4 days | |
| Results | MDA-MB-231 cells were treated with 32nM SN-38 for 4 days and then incubated for 4 days. Most of the MDA-MB-231 cells were transformed into senescent cells. |
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Source | J Immunol Res (2018). Figure 1. SN-38 |
| Method | flow cytometry | |
| Cell Lines | NB cell lines | |
| Concentrations | 1 nM | |
| Incubation Time | 24 hours | |
| Results | Cisplatin at 2 μM, etoposide at 0.1 μM, irinotecan at 1 nM, and topotecan at 10nM induced apoptosis of all NB cell lines after 48 hours of treatment, with the exception of SK-N-AS treated with the first three drugs and SK-N-BE(2)c treated with all four drugs |
| Cell Experiment | |
|---|---|
| Cell lines | A-172, U-87, and LA-567 |
| Preparation method | MTT assay |
| Concentrations | 0 -1000 nM |
| Incubation time | 48 h |
| Animal Experiment | |
|---|---|
| Animal models | Nontumor-bearing mice |
| Formulation | dissolved in DMSO (1 mg/mL) |
| Dosages | 1 mg/kg |
| Administration | i.v. |
| Molecular Weight | 392.4 |
| Formula | C22H20N2O5 |
| CAS Number | 86639-52-3 |
| Solubility (25°C) | DMSO 21 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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