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RS-1

Cat. No. M6148
RS-1 Structure
Size Price Availability Quantity
10mg USD 90 In stock
25mg USD 180 In stock
50mg USD 260 In stock
100mg USD 460 In stock
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Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity.

Protocol
Cell Experiment
Cell lines PC3 cells
Preparation method PC3 cells were treated with 60 μM RS-1 for the indicated times. Cells were collected and fixed in ice cold 70% ethanol for at least 4 hours. Cells were rinsed and stained with 50 μg/ml PI, 50 μg/ml RNAse A in PBS for 20 minutes. Cell cycle distributions were analyzed by flow cytometry using a LSR-II (BD biosciences) cytometer. The percentage of cells in G1, S, and G2/M phases of the cell cycle was determined using FlowJo software.
Concentrations 60 μM
Incubation time 24 h
Animal Experiment
Animal models Athymic nude mice
Formulation 30% DMSO, 35% PEG-400, 35% PBS
Dosages 200 μl
Administration Peritoneal administrations
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 524.23
Formula C20H16Br2N2O3S
CAS Number 312756-74-4
Purity >98%
Solubility 100 mg/mL in DMSO
Storage at -20°C
References

RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency.
Song J, et al. Nat Commun. 2016 Jan 28;7:10548. PMID: 26817820.

The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors.
Mason JM, et al. Cancer Res. 2014 Jul 1;74(13):3546-55. PMID: 24753542.

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  Catalog
Abmole Inhibitor Catalog 2017




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