Resveratrol is a selective human cytochrome P450 1A1 inhibitor. Resveratrol slightly inhibited ethoxyresorufin O-deethylation (EROD) activity in human liver microsomes with an IC50 of 1.1 mM.
|Cell lines||CNE-1 and CNE-2Z cells|
|Preparation method||Cell proliferation assay
The effect of resveratrol on the cell growth of CNE-1 and CNE-2Z cells was examined using the MTT assay. Cancer cells were plated in 96-well plates at 104 cells/well. Following pretreatment with different concentrations of resveratrol for 24 or 48 h, viable cells were determined using the MTT Assay kit (Chemicon, Temecula, CA, USA) according to the manufacturer’s protocol. At indicated time-points, 20 μl of MTT solution (5 mg/ml in PBS) were added into each well and incubated for 4 h. Then, 100 μl of DMSO was added to dissolve the crystals. The plate was then left to stand for 10 min at room temperature and the corresponding absorbance was measured at 490 nm. Each experiment was performed in triplicate.
|Concentrations||0, 10, 25, 50 and 100 μM|
|Incubation time||24 or 48 h|
|Animal models||female nude mice bearing CNE-2Z tumour xenograft model|
|Formulation||10% DMSO in PBS|
|Dosages||20 mg/kg once a day for 3 weeks|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO 40 mg/mL|
Resveratrol inhibits human nasopharyngeal carcinoma cell growth via blocking pAkt/p70S6K signaling pathways.
Zhang M, et al. Int J Mol Med. 2013 Mar;31(3):621-7. PMID: 23314035.
|Related Sirtuin Products|
SRT2104 is a selective brain-permeable activator of SIRT1.
3-TYP is a selective SIRT3 inhibitor, more potent over SIRT1 and SIRT2, with IC50 values of 88 nM, 92 nM, 16 nM for SITR1, SIRT2, SIRT3, respectively.
|Salvianolic acid B
Salvianolic acid B is a cell protective antioxidant and free radical scavenger being investigated for a variety of conditions from ischemic heart disorders to Alzheimer′s disease.
OSS_128167 is a specific SIRT6 inhibitor with IC50 values of 89, 1578 and 751 μM for SIRT6, SIRT1 and SIRT2, respectively.
|Tenovin 6 Hydrochloride
Tenovin-6 Hydrochloride is a water soluble inhibitor of SIRT1 and SIRT2, slightly inhibits HDAC8, and is also a potent activator of p53, with IC50s of 21 μM, 10 μM, 67 μM for SirT1, SirT2, and SirT3, respectively.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.