Expression system: E.coli
Apparent molecular weight: 28 KDa, reducing conditions
Purity: >95% as determined by reduced SDS-PAGE
Endotoxin: <0.1 ng/µg (1 EU/µg) by LAL test
Storage and Stability: Lyophilized proteins should be stored at <-20°C, although stable at room temperature for up to 3 weeks. Reconstituted protein solutions can be stored at 4-7°C for 2-7 days. Recombinant sample aliquots are stable for 3 months at <-20°C.
Bioactivity: Glutathione S-transferases (GSTs), formerly known as ligand proteins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for detoxification purposes.The GST family consists of three superfamilies: cell membrane, mitochondrial, and microsomal (MAPEG) proteins. GST isozymes appear to play a central role in the detoxification system of the parasite. Other functions are also suspected to include a role in increasing the solubility of heme in the parasite gut. the activity of GSTs depends on a steady supply of GSH from the synthases γ-glutamylcysteine synthase and glutathione synthase, as well as the action of specific transporters that remove GSH conjugates from the cell. the main role of GSTs is through the catalysis of GSH on said nonpolar xenobiotic substrates to detoxify xenobiotics by catalyzing the nucleophilic attack of electrophilic carbon, sulfur or nitrogen atoms, thereby preventing them from interacting with critical cellular proteins and nucleic acids.
Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100μg/ml. |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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