Genus: Mouse
Expression system: E. coli
Purity: >95% by SDS-PAGE analysis
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Apparent molecular weight: ~15.2 kDa on SDS-PAGE under non-reducing conditions
Storage and Stability: This product is stable for up to 6 months at temperatures below -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: Interleukin-3 (IL-3) is a polymorphic cytokine belonging to the interleukin family.IL-3 shares similarities with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-5: they both have a four-helix bundle structure, are located on the same chromosome in both humans and mice, are produced by activated T cells, and have a common receptor.IL- IL-3 is also known as a multicolony stimulating factor because it stimulates the development and colony formation of multilineage hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. -2 gene family to inhibit apoptosis and promote cell survival.
Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] A Kusumi, et al. Gan To Kagaku Ryoho. [Interleukin 3 (IL-3)]
Related Recombinant Proteins Products |
---|
Recombinant Mouse E-Selectin/CD62E Protein (HEK293)
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. |
Recombinant Human DPP4/CD26 (Mammalian, C-6His)
DPP4/CD26 is a signal-anchor for type II membrane protein that belongs to the peptidase S9B family. DPP4/CD26 acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. It’s binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. |
Recombinant Human USP14 (E.coli, N-6His)
USP14 belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. |
Recombinant Human EpCAM/Trop-1 (Mammalian, C-Fc)
Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein. EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5, MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells. |
Recombinant Human IL-36 gamma/IL-1 F9 Protein (E. coli, aa 18-169, His Tag)
The recombinant human IL1F9 consists of 168 amino acids and predicts a molecular mass of 19.1 KDa. A DNA sequence encoding the mature form of human IL1F9 (Ser18-Asp169) was expressed with a polyhistide tag at the N-terminus. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.