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Recombinant Mouse GM-CSF Protein (E. coli)

Cat. No. M11485
Recombinant Mouse GM-CSF Protein (E. coli) Structure
Synonym:

Granulocyte Macrophage Colony Stimulating Factor; CSF-2; MGI-1GM

Size Price Availability Quantity
10ug USD 177  USD177 In stock
50ug USD 432  USD432 In stock
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Quality Control & Documentation
  • Purity: >98%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Purity :> 98% SdS-PAGE analysis, HPLC analysis of 98%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~14.3 kDa, reduced by SDS-PAGE.
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Bioactivity: Granulocyte macrophage colony stimulating factor (GM-CSF) was originally thought to be a growth factor capable of supporting granulocyte - macrophage progenitor cell colony formation in vitro. Granulocyte macrophage colony stimulating factor (GM-CSF) is produced by many different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to immune and inflammatory cytokines. In addition, granulocyte macrophage colony stimulating factor (GM-CSF) stimulates the proliferation of a variety of tumor cell lines, including osteosarcoma, carcinoma, and adenocarcinoma cell lines.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH₂O or PBS to 100 μg/ml
Storage Stable for up to 6 months at lower than -70°C
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Elektra Kantzari Robinson, et al. PLoS One. Generation and utilization of a HEK-293T murine GM-CSF expressing cell line

[2] Michael Dougan, et al. Immunity. GM-CSF, IL-3, and IL-5 Family of Cytokines: Regulators of Inflammation

[3] Marco Grasse, et al. Vaccine. GM-CSF improves the immune response to the diphtheria-component in a multivalent vaccine

[4] Qingjie Xue, et al. Hum Vaccin Immunother. Efficacy of recombinant adenovirus expressing a fusion gene from GM-CSF and Epstein-Barr virus LMP2A in a mouse tumor model

[5] B L Liu, et al. Gene Ther. ICP34.5 deleted herpes simplex virus with enhanced oncolytic, immune stimulating, and anti-tumour properties

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Keywords: Recombinant Mouse GM-CSF Protein (E. coli), Granulocyte Macrophage Colony Stimulating Factor; CSF-2; MGI-1GM supplier, Recombinant Proteins, inhibitors, activators


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