Purity :> 98% SdS-PAGE analysis, HPLC analysis of 98%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~14.3 kDa, reduced by SDS-PAGE.
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: Granulocyte macrophage colony stimulating factor (GM-CSF) was originally thought to be a growth factor capable of supporting granulocyte - macrophage progenitor cell colony formation in vitro. Granulocyte macrophage colony stimulating factor (GM-CSF) is produced by many different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to immune and inflammatory cytokines. In addition, granulocyte macrophage colony stimulating factor (GM-CSF) stimulates the proliferation of a variety of tumor cell lines, including osteosarcoma, carcinoma, and adenocarcinoma cell lines.
Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O or PBS to 100 μg/ml |
Storage | Stable for up to 6 months at lower than -70°C |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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