Species: Mouse
Expression system: CHO
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Purity: > > 95% by SDS-PAGE, > > > 95% by HPLC
Apparent molecular weight: 17~22 kDa on SDS-PAGE under reducing conditions
Storage and Stability: This product is stable for up to 6 months at less than -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: It is expressed in both central nervous system (CNS) and non-CNS tissues.GDNF signals through a receptor system consisting of RET and one of the four GFRα receptors. It promotes the survival and differentiation of dopaminergic neurons and increases their high affinity for dopamine uptake. In mouse models of Parkinson's disease, GDNF has been shown to improve motor bradykinesia, rigidity and postural instability.GDNF has also been shown to regulate kidney development, spermatogenesis and influence alcohol consumption.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[5] Jun Kawanokuchi, et al. J Neuroimmunol. Production and functions of IL-17 in microglia
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