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Recombinant Human TNF-β (E. coli)

Cat. No. M21057
Recombinant Human TNF-β (E. coli) Structure
Synonym:

LTA; LT; TNFB; TNFSF1

Size Price Availability Quantity
10ug USD 148  USD185 In stock
50ug USD 304  USD380 In stock
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Quality Control
  • Current batch:
  • Purity >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Genus: Human
Expression system: E. coli
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Purity: >95% by SDS-PAGE analysis
Apparent molecular weight: ~18.6 kDa on SDS-PAGE under reducing conditions
Storage and Stability: This product is stable for up to 6 months at temperatures below -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Bioactivity: TNF is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells upon stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α, while CD8 cells secrete little or no TNF-α. TNF-α synthesis is induced by many different stimuli, including interferon, IL2, and GM-CSF. TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes and has similar activity to TNF-α. It mediates a large number of inflammatory, immunostimulatory and antiviral responses.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity >95% , Endotoxin < 0.2 EU/μg
Solubility Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage at -20°C
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Keywords: Recombinant Human TNF-β (E. coli), LTA; LT; TNFB; TNFSF1 supplier, Cytokines, inhibitors

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