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Recombinant Human LIF Protein (E.coli)

Cat. No. M11370
Recombinant Human LIF Protein (E.coli) Structure
Synonym:

Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor

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50ug USD 465  USD465 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin ≤0.01EU/μg
  • COA
  • MSDS
Biological Activity

Expression host: E.coli
Species: Human
Endotoxin: less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL.
Purity: > 95% as determined by reduced SDS-PAGE
Storage Conditions: Lyophilized proteins should be stored at < -20°C, but at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.


Biological activity: TF-1 human erythroid leukemia cells were measured in cell proliferation assays. The ED50 of this effect is 25-150 pg/ mL. Leukemia suppressor factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. LIF also has a variety of activities such as cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitosis of some factor dependent cell lines, and promotion of megakaryocyte production in vivo. Human and mouse mature LIFs have 78% sequence homology at the amino acid level. Human LIF is also active in human and mouse cells. Mouse LIF is about 1000 times more active against human cells than human LIF.

Chemical Information
Solubility (25°C) It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Hee Jung Son, et al. Spine J. Efficacy and safety of Escherichia coli-derived recombinant human bone morphogenetic protein-2 in additional lumbar posterolateral fusion: minimum 1-year follow-up

[2] Amelie Croset, et al. J Biotechnol. Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells

[3] Christa Broholm, et al. J Appl Physiol (1985). LIF is a contraction-induced myokine stimulating human myocyte proliferation

[4] A Jurisicova, et al. Fertil Steril. Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation

[5] D P Gearing, et al. Ciba Found Symp. Reconstitution of high affinity leukaemia inhibitory factor (LIF) receptors in haemopoietic cells transfected with the cloned human LIF receptor

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Keywords: Recombinant Human LIF Protein (E.coli), Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor supplier, Recombinant Proteins, inhibitors, activators


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