Interleukin-8 (IL-8), also known as CXCL8, exists in two forms: a 77 a.a. protein produced by endothelial cells, and the more active 72 a.a. protein produced by monocytes in vivo. Il-8 receptors are 7 helical G protein-coupled receptors CXCR1 and CXCR2 and are expressed only on neutrophils. The function of IL-8 is to induce rapid changes in cell morphology, activate integrins, and release neutrophil particle content. Therefore, IL-8 can enhance the antibacterial action of defense cells. It is secreted by monocytes, macrophages and endothelial cells. Il-8 signaling attracts neutrophils, basophils, and T cells via CXCR1 and CXCR2. Il-8 is also a potent promoter of angiogenesis.
The EC50 value of human IL-8(77aa) on Ca2+ mobilization assay in CHO-K1/G15/hCXCR1 cells is less than 150.0 ng/ml.
Accession: P10145
Endotoxin < 0.2 EU/μg
Apparent Molecular Weight: ~8.9 kDa, reducing conditions
Lyophilized after extensive dialysis against PBS.
Form | Lyophilized powder |
Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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