The role of Recombinant Human IL-4 Protein (CHO) in the induction of monocytes into DCs is to inhibit the overgrowth of macrophages, thereby directing monocyte differentiation toward DCs. Also, IL-4 has the ability to reduce cell surface expression of CD14 molecules, an important marker for the differentiation of monocytes into DCs. The combined effect of GM-CSF and IL-4 can lead to targeted differentiation of monocytes into immature DCs, which have a strong capacity for antigen uptake and processing, but a weak capacity for antigen presentation.
DNA sequence encoding human IL-4 ( GenBank: AAH70123.1 H25-S153 ) expressed with an Fc tag at the C- terminus.
Measured by the cell proliferation assay with TF-1 cells, the ED50 is ≤0.5 ng/mL.
Endotoxin <0.1 EU/μg
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at ≤-20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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