Recombinant Human IL-13 Protein (HEK293) is an immunomodulatory cytokine, mainly produced by activated Th2 cells, mast cells and NK cells. Interleukin-13 plays an important role in the elimination of gastrointestinal parasites. rhIL-13 has anti-inflammatory effects on monocytes and macrophages, and inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to promote B cells Proliferate and induce isoform conversion, thereby increasing the production of IgE. Blocking IL-13 activity can inhibit the pathophysiology of asthma. Human and mouse IL-13 are cross-species responses. Recombinant human IL-13 contains 118 amino acids with a predicted molecular weight of 13.2kd.
Measured by dose-dependent proliferation of TF-1 cells, the ED50 value is ≤1.0ng/ml, corresponding specific activity is ≥1×106 U/mg.
Protein Sequence: DNA sequence encoding human IL-13 (GenBank: U31120.1) expressed with a His tag at the C-terminus.
Endotoxin: <0.1EU/μg (gel method)
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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