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Recombinant Human FGF-21 (E. coli)

Cat. No. M15030
Recombinant Human FGF-21 (E. coli) Structure
Synonym:

FGF21; fibroblast growth factor-21; FGFL

Size Price Availability Quantity
10ug USD 180  USD180 In stock
50ug USD 360  USD360 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: E. coli
Purity: SDS-PAGE analysis, > 95%, HIGH performance liquid chromatography analysis, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~19.5 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Bioactivity: ED50 < 0.5µg/ mL, niH-3T3 cells were used for cell proliferation assay in the presence of mouse Klotho 1.25µg/ mL and heparin 10µg/ mL, corresponding specific activity > 2.0 × 103 units /mg. Along with FGF-19/15 and FGF-23, FGF-21 is classified as a member of the atypical FGF-subfamily because it must bind to the Klotho coreceptor in order to bind to the FGF-receptor and activate downstream signaling pathways. In vivo, FGF-21 is expressed in liver, pancreas, adipose tissue and skeletal muscle and plays a central role in energy metabolism. Fgf-21 expression is stimulated by free fatty acids and insulin resistance status, and is associated with systemic insulin resistance. Fgf-21 activates glucose uptake by fat cells and increases insulin sensitivity, suggesting that it is a new target with potential anti-diabetic properties.

Chemical Information
Solubility (25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Xianlong Ye, et al. Int J Biol Macromol. An efficient large-scale refolding technique for recovering biologically active recombinant human FGF-21 from inclusion bodies

[2] Qi Hui, et al. Appl Microbiol Biotechnol. Two-hundred-liter scale fermentation, purification of recombinant human fibroblast growth factor-21, and its anti-diabetic effects on ob/ob mice

[3] Xianlong Ye, et al. J Biotechnol. Pilot-scale production and characterization of PEGylated human FGF-21 analog

[4] Yu-Ting Hou, et al. Yi Chuan. [Cloning, expression and glucose regulation activity of human FGF-21]

[5] Gui-ping Ren, et al. Yao Xue Xue Bao. [Efficient expression of soluble human FGF-21 and its glucose regulation activity]

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Keywords: Recombinant Human FGF-21 (E. coli), FGF21; fibroblast growth factor-21; FGFL supplier, Recombinant Proteins, inhibitors, activators


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