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Recombinant Human EGF Protein (HEK293, C-His)

Cat. No. M9415
Recombinant Human EGF Protein (HEK293, C-His) Structure
Size Price Availability Quantity
50ug USD 175  USD175 In stock
100ug USD 320  USD320 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.1 EU/μg
  • COA
  • MSDS
Biological Activity

Recombinant Human EGF Protein (HEK293) is an important class of growth factors. EGF is widely used as a mitogen in the in vitro culture of neural stem cells and can be used as an inducer to promote the differentiation of neural stem cells into neurons, astrocytes, and oligodendrocytes.

Measured by cell proliferation assay with Balb/c 3T3 cells, the ED50 is ≤3 ng/mL.


Molecular Weight: ~7.0 kD

Endotoxin <0.1EU/μg

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml.
Storage Stored at ≤-20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

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