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Recombinant Human CYP3A4 protein (E.coli)

Cat. No. M11363
Recombinant Human CYP3A4 protein (E.coli) Structure
Size Price Availability Quantity
50ug USD 300  USD300 In stock
100ug USD 440  USD440 In stock
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Quality Control & Documentation
Biological Activity

Purity: sdS-PAGE determination was >90%
Protein construction: The DNA sequence encoding human CYP3A4 (Gly26-Ala503) was fused with His marker
Predicted molecular weight: 52.98 kDa
Stability and storage: use manual defrosting refrigerator to avoid repeated freeze-thaw cycles.
Store at 2-8 °C for one week.
Store at -20 to -80°C for 12 months from date of receipt.

CYP3A4 is an important member of the cytochrome P45 enzyme superfamily, with 33 alleles. Human cytochrome P45 3A4 (CYP3A4) is an important drug metabolism enzyme involved in many drug-drug and food-drug interactions. Systemic inflammation is associated with inhibition of CYP3A4 activity.


Chemical Information
Solubility (25°C) N/A
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Chen Sun, et al. Drug Metab Dispos. Icotinib Induces Mechanism-Based Inactivation of Recombinant Human CYP3A4/5 Possibly via Heme Destruction by Ketene Intermediate

[2] Yaoyao Cai, et al. Chem Res Toxicol. Evaluation of Recombinant CYP3A4 Variants on the Metabolism of Oxycodone In Vitro

[3] Marie Yamada, et al. J Microbiol Biotechnol. Detoxification of Aflatoxin B1 Contaminated Maize Using Human CYP3A4

[4] Bingbing Chen, et al. Chem Res Toxicol. Effects of 26 Recombinant CYP3A4 Variants on Brexpiprazole Metabolism

[5] Irina Sevrioukova. Biochemistry. Interaction of Human Drug-Metabolizing CYP3A4 with Small Inhibitory Molecules

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