Free shipping on all orders over $ 500

Recombinant Human CD25/IL-2Rα Fc Chimera (HEK 293)

Cat. No. M15034
Recombinant Human CD25/IL-2Rα Fc Chimera (HEK 293) Structure
Synonym:

CD25 antigen; CD25; IDDM10

Size Price Availability Quantity
10ug USD 350  USD350 In stock
50ug USD 990  USD990 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: HEK 293
Purity: SDS-PAGE, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: 65~ 75kDa, reduced on SDS-PAGE
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Bioactivity: 5.0 μg/ mL (100 μ L/well) immobilized human IL-2 can bind to CD25/IL-2Rα, hFc and human IL-2Rα. Activation T cells, regulatory T cells (Tregs) and NK cells expressed CD25 at high levels, and the expression of high-affinity IL-2Rα was mainly confined to these cell populations. The il-2R α signaling pathway mediates a variety of biological processes in various cell populations, such as proliferation and differentiation of B cells and NK cells. A soluble form of IL-2Rα (IL-2Rα) is present in serum with increased expression on cells. The function of soluble IL-2Rα is unclear. Increased il-2R α levels in biological fluids have been reported to be associated with increased T and B cell activation and immune system activation. Serum il-2R α concentrations are elevated in many inflammatory processes and in some leukemias and lymphomas.

Chemical Information
Solubility (25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Related Recombinant Proteins Products
Recombinant Human IL-10 (CHO)

Interleukin-10 (IL-10) is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and Th2 cells.

Recombinant Human IL-5 (CHO)

Interleukin-5 (IL-5) is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors.

Recombinant Human Tau-F Protein (E.coli)

Tau proteins are proteins which contain four Tau/MAP repeats. They promote microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity.

Recombinant Human Tau Protein (E. coli, His Tag)

Tau proteins are proteins that stabilize microtubules. Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability of axonal microtubules.

Recombinant Rat IL-23 Protein (HEK293, C-His)

IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Human CD25/IL-2Rα Fc Chimera (HEK 293), CD25 antigen; CD25; IDDM10 supplier, Recombinant Proteins, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.