PDTC can enhance the cell apoptosis induced by TNF-α, which may be associated with the blocking of TNF-α-activated NF-κB signaling pathway by PDTC, the down-regulation of survivin expression, and up-regulation of caspase-3 expression.in NMSC PDTC acts as pro-oxidant and enhances IR-induced cell cytotoxicity by increasing H(2)O(2)formation and thiol oxidation.PDTC and MG132, inhibitors of NF-kappaB, block ET-induced vasodilation in isolated rat skeletal muscle arterioles.PDTC has effects at both the level of the aortic segment as well as the resistance arteriole.PDTC prevents induction of nitric oxide synthase (NOS) by inhibiting translation of NOS mRNA.PDTC induces apoptosis in rat smooth muscle cells and inhibits apoptosis in leukemia HL-60 cells.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Source||J Infect Dis (2018). Figure 7. PDTC (Abmole Bioscience)|
|Cell Lines||mast cells|
|Incubation Time||30 minutes|
|Results||The S. aureus-induced production of TNF-α and CRAMP was significantly reduced by pretreatment with the specific PI3K inhibitor Wortmannin or the specific NF-κB inhibitor PDTC|
|Source||Sci Rep (2017). Figure 4. PDTC (AbMole BioScience Inc., Brussels, Belgium)|
|Method||Inhibitors of innate signalling pathways|
|Cell Lines||HEK cells|
|Incubation Time||24 h|
|Results||As expected, RELA knock-down and the presence of PDTC, an inhibitor of NFκB, resulted in substantial reduction of CXCL8 production in response to H7 flagellin by hTLR5 in the HEK background (p < 0.05)|
|Source||Vete Mic (2017). Figure 4. PDTC (Abmole Bioscience, Houston, TX, USA)|
|Method||Treatment by inhibitors|
|Incubation Time||48 h|
|Results||The PDTC (NF-kB inhibitor) treatment significantly decreased the MHC II, CD80, CD86, CD40 expressions. As to cytokine production (Fig. 4e–h), both inhibitors significantly restrained TNF-a, IFN-g and IL-6 secretions, with a minimal effect on IL-12p70.|
J Infect Dis. 2018 May 8.
Sci Rep. 2017 Dec 15;7(1):17677.
Host species adaptation of TLR5 signalling and flagellin recognition
PDTC purchased from AbMole
Vet Microbiol. 2017 May;203:158-166.
[NF-κb inhibitor PDTC enhances tumor necrosis factor α-induced apoptosis of gastric cancer cell SGC-7901].
Cao MZ, et al. Zhonghua Wei Chang Wai Ke Za Zhi. 2013 Jun;16(6):578-82. PMID: 23801215.
Pyrrolidine dithiocarbamate (PDTC) blocks apoptosis and promotes ionizing radiation-induced necrosis of freshly-isolated normal mouse spleen cells.
Thompson JS, et al. Apoptosis. 2010 Jun;15(6):705-14. PMID: 20333468.
PDTC and Mg132, inhibitors of NF-kappaB, block endotoxin induced vasodilation of isolated rat skeletal muscle arterioles.
Snyder JG, et al. Shock. 2002 Apr;17(4):304-7. PMID: 11954831.
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5-Aminosalicylic acid is a specific inhibitor of TNFα-induced IKK activity, used to treat inflammatory bowel disease.
GSK583 is a highly potent and selective inhibitor of RIP2 kinase with an IC50 of 5 nM.
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