Free shipping on all orders over $ 500


Cat. No. M1809
NU7441 Structure

KU 57788

Size Price Availability Quantity
Free Sample 0.5 mg  USD 0 In stock
5mg USD 65  USD65 In stock
10mg USD 100  USD100 In stock
50mg USD 330  USD330 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

NU7441 (KU 57788) is a potent and selective DNA-dependent protein kinase (DNA-PK) inhibitor with IC50 values of 14, 1700, 5000, >100000 and >100000 nM for DNA-PK, mTOR, PI 3-K, ATM and ATR respectively. NU7441 strongly inhibited DNA-PK in cell lines (IC(50) = 0.3 μM) but only weakly inhibited PI3 K (IC(50) = 7 μM). The only available anti-phospho-DNA-PK antibody also recognised some phosphoprotein targets of ATM. NU7441 (KU 57788) caused doxorubicin- and IR-induced DNA DSBs (measured by γ-H2AX foci) to persist and also slightly decreased homologous recombination activity, as assessed by Rad51 foci. Chemo- and radio-potentiation were induced by NU7441 in M059-Fus-1, but not in DNA-PK-deficient M059 J cells. DNA-PK was highly expressed in a chronic lymphocytic leukaemia sample but undetectable in resting normal human lymphocytes, although it could be induced by PHA-P treatment. In K652 cells, DNA-PK expression was not related to cell cycle phase. NU7441 increased etoposide-induced tumor growth delay 2-fold without exacerbating etoposide toxicity to unacceptable levels. In conclusion, NU7441 (KU 57788) shows sufficient proof of principle through in vitro and in vivo chemosensitization and radiosensitization to justify further development of DNA-PK inhibitors for clinical use.

Product Citations
Customer Product Validations & Biological Datas
Source bioRxiv (2020 Mar) Figure 6. NU7441 (Abmole Bioscience, Houston, TX, USA)
Method BLRR assay
Cell Lines BLRR cells
Concentrations 2×10^-3 M
Incubation Time 1 h
Results Following NU7441 treatment, the Gluc signal increased as the Vluc signal decreased in a dose-dependent manner.
Source Breast Cancer Res Treat (2014). Figure 2. NU7441
Method Western analysis
Cell Lines human breast cancer cells
Concentrations 1 μM
Incubation Time 1 h
Results "Repair was slower in the other two cell lines, which took approximately 18 h to repair 90 % of the breaks. NU7441 retarded the repair in all three cell lines with the greatest effect being observed in MCF-7 cells , where 20 % of DSBs persisted for at least 24 h."
Cell Experiment
Cell lines SW620, LoVo, V3, and V3-YAC cells
Preparation method Cytotoxicity and growth inhibition studies.
We exposed exponentially growing cells in six-well plates or 6-cm dishes to etoposide or doxorubicin with or without NU7441 (0.5 or 1.0 μmol/L) for 16 hours. For radiosensitization studies, NU7441 was added to the cells 1 hour before irradiation. V3 and V3-YAC cells were exposed to γ-irradiation (3.1 Gy/min 137Cesium, Gammacell 1000 Elite, Nordion International Ltd., Ottawa, Ontario, Canada). SW620 and LoVo were exposed to X-irradiation (2.9 Gy/min at 230 kV, 10 mA; Gulmay Medical Ltd., Camberly, United Kingdom) due to the equipment available. After irradiation, the cells were incubated with or without NU7441 for a further 16 hours. Cells were then harvested by trypsinization, counted, and seeded into 10-cm diameter Petri dishes at densities varying from 100 to 100,000 per dish in drug-free medium for colony formation. Cell growth inhibition following 5-day continuous exposure to NU7441 was determined in LoVo and SW620 cells grown in 96-well plates as described previously.
Concentrations 0, 2, 4, 6, 8μ M
Incubation time 5 days
Animal Experiment
Animal models CD-1 nude mice bearing palpable SW620 colorectal cancer xenografts
Formulation dissolved in 40% PEG 400 in saline
Dosages 10 mg/kg
Administration i.p.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 413.49
Formula C25H19NO3S
CAS Number 503468-95-9
Purity >99%
Solubility DMSO 10 mg/mL
Storage at -20°C

[1] Zhao Y, et al. Cancer Res. Preclinical evaluation of a potent novel DNA-dependent protein kinase inhibitor NU7441.

Related PI3K Products

Gdc-0077 (RG6114) is an effective, oral, selective PI3Kα inhibitor (IC50=0.038 nM). Gdc-0077 (RG6114) exerts its activity by binding to the ATP binding site of PI3K, thereby inhibiting phosphorylation of PIP2 to PIP3. Compared with wild-type PI3Kα, GDC-0077 (RG6114) showed higher selectivity against mutants.


Zandelisib (ME-401) is a PI3K inhibitor that selectively inhibits P110 δ with an IC50 value of 3.5 nM. For details, please refer to compound 1 in patent literature WO2019183226 A1. Zandelisib has antitumor effects.

Quercetin (97%)

Quercetin is a PI3K inhibitor with IC50 of 2.4-5.4 μM that has been shown to cause potent reversible inhibition of fatty-acid synthase.

Erucic acid

Erucic acid is a monounsaturated fatty acid (MUFA) isolated from radish seeds. Erucic acid, which easily crosses the blood-brain barrier (BBB), normalizes the accumulation of long-chain fatty acids in the brain.

Linperlisib (YY-20394)

Linperlisib, also known as YY-20394 and PI3Kδ-IN-2, is a potent and selective PI3Kδ inhibitor.

Abmole Inhibitor Catalog 2017

Keywords: NU7441, KU 57788 supplier, PI3K, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.