NSC23766 3HCl is an inhibitor of Rac GTPase targeting Rac activation by guanine nucleotide exchange factors (GEFs) with IC50 of ~50 μM; does not inhibit the closely related targets, Cdc42 or RhoA.
bioRxiv. 2021 Apr 26.
Self-organization of Tissue Growth by Interfacial Mechanical Interactions in Multi-layered Systems
NSC23766 trihydrochloride purchased from AbMole
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Source | bioRxiv 2021. Figure 1. NSC23766 (Abmole Bioscience, USA) |
| Method | Immunofluorescence | |
| Cell Lines | Hela human cervical carcinoma cell、Madin-Darby canine kidney (MDCK) cells、 MDA-MB-231 human breast cancer cell、U2OS human osteosarcoma cell | |
| Concentrations | 20 µM | |
| Incubation Time | 48 hours | |
| Results | Progressive compression gradient is strengthened in epidermal layer during chicken feather follicle morphogenesis |
| Molecular Weight | 530.96 |
| Formula | C24H35N7.3HCl |
| CAS Number | 1177865-17-6 |
| Solubility (25°C) | Water: ≥ 25 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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