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Nitrotetrazolium Blue chloride

Cat. No. M9793
Nitrotetrazolium Blue chloride Structure
Synonym:

NBT

Size Price Availability Quantity
100mg USD 40 In stock
500mg USD 80 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

Nitrotetrazolium Blue chloride (NBT) is a substrate for dehydrogenases, used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 817.64
Formula C40H30Cl2N10O6
CAS Number 298-83-9
Purity >98%
Solubility Water 10 mg/mL
Storage 2-8°C, protect from light, dry, sealed
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Nitrotetrazolium Blue chloride, NBT supplier, Fluorescent Dye, inhibitors

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