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NBD-Cl

Cat. No. M13795
NBD-Cl Structure
Synonym:

NBD chloride

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Biological Activity

NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.

Chemical Information
Molecular Weight 199.55
CAS Number 10199-89-0
Solubility (25°C) DMSO ≥ 90 mg/mL
Storage 2-8°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Hend Aly, et al. RSC Adv. Utilization of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) for spectrochemical determination of l-ornithine: a multivariate optimization-assisted approach

[2] Kohei Watanabe, et al. Chem Sci. Reactions of an anionic chelate phosphane/borata-alkene ligand with [Rh(nbd)Cl] 2, [Rh(CO) 2 Cl] 2 and [Ir(cod)Cl] 2

[3] Mahmoud A Omar, et al. Luminescence. New spectrofluorimetric analysis of dapagliflozin after derivatization with NBD-Cl in human plasma using factorial design experiments

[4] Ana L Prez, et al. Spectrochim Acta A Mol Biomol Spectrosc. A novel fluorimetric method for glyphosate and AMPA determination with NBD-Cl and MCR-ALS

[5] Cornelia Hunke, et al. J Bioenerg Biomembr. The effect of NBD-Cl in nucleotide-binding of the major subunit alpha and B of the motor proteins F1FO ATP synthase and A1AO ATP synthase

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