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Monomethyl auristatin E

Cat. No. M3741
Monomethyl auristatin E Structure
Synonym:

MMAE, Vedotin

Size Price Availability Quantity
1mg USD 90 In stock
5mg USD 210 In stock
10mg USD 350 In stock
50mg USD 980 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Monomethyl auristatin E (MMAE; Vedotin) is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. Monomethyl auristatin E (MMAE), linked via the lysosomally cleavable dipeptide, valine-citrulline (vc). Antibody-drug conjugates (ADCs) are designed to combine the exquisite specificity of antibodies to target tumor antigens with the cytotoxic potency of chemotherapeutic drugs. In vivo studies demonstrated that the peptide-linked conjugates induced regressions and cures of established tumor xenografts with therapeutic indices as high as 60-fold.

Protocol
Cell Experiment
Cell lines CD30+ Karpas 299 cells
Preparation method Using Alamar Blue dye reduction assay to measure cytotoxicity according to the manufacturer's directions. Briefly, freshly preparing a 40% solution (wt/vol) of Alamar Blue in complete media just before adding cultures . Ninety-two hours after drug exposure, adding Alamar Blue solution to cells to constitute 10% culture volume. Incubating cells for 4 hours, and measuring dye reduction on a Fusion HT fluorescent plate reader (Packard Instruments, Meriden, CT).
Concentrations ~1000 ng/mL
Incubation time 96 h
Animal Experiment
Animal models SCID mice bearing Karpas 299 (ALCL) or L540cy (HD) tumors
Formulation
Dosages 0.36 mg/kg
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 717.98
Formula C39H67N5O7
CAS Number 474645-27-7
Purity >99%
Solubility DMSO >100 mg/mL
Storage at -20°C
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Monomethyl auristatin E, MMAE, Vedotin supplier, Microtubule, inhibitors

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