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MitoTracker Green FM

Cat. No. M19978
MitoTracker Green FM Structure
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50ug USD 100  USD100 In stock
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Biological Activity

Mitotracker green FM is a cell permeable carbocyanine structural derivative containing a weakly thiol reactive chloromethyl functional group that marks mitochondria. This product is a bright green fluorescent probe ((ex = 490 nm, EM = 516 nm)). It hardly fluoresces in aqueous solution. It can only fluoresce when it is concentrated in the lipid environment of mitochondria, and the background fluorescence is almost negligible. The dye is unstable after acetaldehyde fixation, so it is more suitable for living cell staining.

Chemical Information
Molecular Weight 671.8797
Formula C34H28Cl5N3O
CAS Number 201860-17-5
Solubility (25°C) DMSO: Soluble
Storage -20°C, protect from light, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Dajun Liu, et al. Cell Death Dis. c-MYC-induced long noncoding RNA MEG3 aggravates kidney ischemia-reperfusion injury through activating mitophagy by upregulation of RTKN to trigger the Wnt/β-catenin pathway

[2] Namrata Gautam, et al. Methods. A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization

[3] Daniel Puleston. Cold Spring Harb Protoc. Detection of Mitochondrial Mass, Damage, and Reactive Oxygen Species by Flow Cytometry

[4] W Pendergrass, et al. Cytometry A. Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

[5] Luis F Marques-Santos, et al. Biosci Rep. Mitotracker green is a P-glycoprotein substrate

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