Mitotracker green FM is a cell permeable carbocyanine structural derivative containing a weakly thiol reactive chloromethyl functional group that marks mitochondria. This product is a bright green fluorescent probe ((ex = 490 nm, EM = 516 nm)). It hardly fluoresces in aqueous solution. It can only fluoresce when it is concentrated in the lipid environment of mitochondria, and the background fluorescence is almost negligible. The dye is unstable after acetaldehyde fixation, so it is more suitable for living cell staining.
Molecular Weight | 671.8797 |
Formula | C34H28Cl5N3O |
CAS Number | 201860-17-5 |
Solubility (25°C) | DMSO: Soluble |
Storage | -20°C, protect from light, dry, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[5] Luis F Marques-Santos, et al. Biosci Rep. Mitotracker green is a P-glycoprotein substrate
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