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MI-2

Cat. No. M3639

MI-2 Structure

Synonym: MALT1 inhibitor

Size Price Availability Quantity
10mg USD 170 In stock
50mg USD 560 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

MI-2 binds directly to MALT1 and irreversibly suppresses protease function. MI-2 decreases NF-κB activity induced by MALT1. MI-2 inhibits cell proliferation and MALT1-mediated cleavage activity. MI-2 concentrates within human ABC-DLBCL cells and irreversibly inhibited cleavage of MALT1 substrates. MI-2 displayes selective activity against ABC-DLBCL cell lines in vitro and xenotransplanted ABC-DLBCL tumors in vivo.

Protocol
Cell Experiment
Cell lines MLL-AF9 and E2A-HLF transduced murine BMC
Preparation method Plating the MLL-AF9 and E2A-HLF transduced murine BMC in 12-well plates at the concentration of 5×103 cells/mL with 1 mL methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds.After 6 days staining colonies with 100 μL iodonitrotetrazolium chloride at final concentration of 1 mg/mL, incubating at 37°C for 30 min and counting . To replate for the 2nd round, counting colonies at day 6 without staining and washing cells out by 1×PBS buffer and resuspending in IMDM medium which contains 15% FBS, 1% penicillin/streptomycin and IL-3.Plateing 5×103 cells in 12-well plates with 1ml methycellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later staining and counting colonies .
Concentrations ~20 μM
Incubation time 12 days
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 455.72
Formula C19H17Cl3N4O3
CAS Number 1047953-91-2
Purity >99%
Solubility DMSO
Storage at -20°C
References

MALT1 small molecule inhibitors specifically suppress ABC-DLBCL in vitro and in vivo.
Fontan L, et al. Cancer Cell. 2012 Dec 11;22(6):812-24. PMID: 23238016.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: MI-2, MALT1 inhibitor supplier, MALT1, inhibitors

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