Expression system: E. coli
Purity: SDS-PAGE > 95%
Apparent molecular weight: ~8.7 kDa, reduced by SDS-PAGE
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Biological activity: CCL2（E. coli）, also known as monocyte chemotactic and activator (MCAF), was originally purified independently by two groups based on its chemotactic ability to attract monocytes. After its cloning and sequencing, it became clear that the protein was also identical to the product of the human JE gene. Je gene was first discovered in mouse fibroblasts and is a platelet-derived growth factor (PDGF) -induced gene. Human CCL2 cDNA encodes a precursor protein with 99 amino acid residues and a hydrophobic signal peptide with 23 residues, which is cleaved to produce a mature protein with 76 residues. The natural CCL2 is heterogeneous in size due to the addition of linking carbohydrate and sialic acid residues. In addition to fibroblastsI ¾ tumor cells, smooth muscle cells, endothelial cells and mononuclear phagocytes can also constitutively produce CCL2 or under various stimuli. CCL2 is a member of the β (CC) subfamily of chemokines. Recently, the existence of MCP2 and MCP3 with 62% and 73% homology to CCL2 amino acids has been reported.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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