Macrophage colony stimulating factor (M-CSF), also known as colony stimulating factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, and can stimulate the proliferation and movement of macrophages. M-csf is mainly produced by monocytes, macrophages, fibroblasts and endothelial cells. The interaction of M-CSF with its receptor C-FMS is associated with growth, invasion, and metastasis in a variety of diseases, including breast cancer and endometrial cancer.
Measured in a cell proliferation assay using Murine M-NFS-60 cells, the ED50 is < 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 units/mg.
Accession # P07141
Protein Construction: M-CSF (Lys33-Pro187)
Endotoxin: < 0.2 EU/μg
Apparent Molecular Weight: ~30 kDa
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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