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Recombinant Mouse M-CSF Protein (CHO)

Cat. No. M11486
Recombinant Mouse M-CSF Protein (CHO) Structure
Synonym:

CSF1; CSF-1; MCSF

Size Price Availability Quantity
10ug USD 188  USD188 In stock
50ug USD 580  USD580 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Macrophage colony stimulating factor (M-CSF), also known as colony stimulating factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, and can stimulate the proliferation and movement of macrophages. M-csf is mainly produced by monocytes, macrophages, fibroblasts and endothelial cells. The interaction of M-CSF with its receptor C-FMS is associated with growth, invasion, and metastasis in a variety of diseases, including breast cancer and endometrial cancer.

Measured in a cell proliferation assay using Murine M-NFS-60 cells, the ED50 is < 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 units/mg.


Accession # P07141

Protein Construction: M-CSF (Lys33-Pro187)

Endotoxin: < 0.2 EU/μg

Apparent Molecular Weight: 35~44 kDa

Lyophilized after extensive dialysis against PBS.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Jiawei Ji, et al. Int Immunopharmacol. M-CSF and prostratin induced Mregs promote immune tolerance in transplanted mice through Arg-1 pathway

[2] Carolina Duarte, et al. Biochem Biophys Res Commun. Age-dependent effects of the recombinant spike protein/SARS-CoV-2 on the M-CSF- and IL-34-differentiated macrophages in vitro

[3] Chia-Sing Lu, et al. J Hematol Oncol. Oct4 promotes M2 macrophage polarization through upregulation of macrophage colony-stimulating factor in lung cancer

[4] Lauar de Brito Monteiro, et al. Immunobiology. M-CSF- and L929-derived macrophages present distinct metabolic profiles with similar inflammatory outcomes

[5] B D Chen. Blood. In vivo administration of recombinant human interleukin-1 and macrophage colony-stimulating factor (M-CSF) induce a rapid loss of M-CSF receptors in mouse bone marrow cells and peritoneal macrophages: effect of administration route

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Keywords: Recombinant Mouse M-CSF Protein (CHO), CSF1; CSF-1; MCSF supplier, Recombinant Proteins, inhibitors, activators


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