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Ketorolac

Cat. No. M3471
Ketorolac Structure
Size Price Availability Quantity
1g USD 120 In stock
5g USD 420 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Ketorolac (Toradol) is a non-selective COX inhibitor with IC50 of 31.5 µM and 60.5 µM for human recombinant COX-1 and COX-2, respectively. Ketorolac tromethamine is a non-steroidal anti-inflammatory agent (NSAID) in the family of heterocyclic acetic acid derivative, often used as an analgesic, antipyretic (fever reducer), and anti-inflammatory. Ketorolac tromethamine (Toradol) acts by inhibiting the bodily synthesis of prostaglandins. The primary mechanism of action responsible for ketorolac’s anti-inflammatory, antipyretic and analgesic effects is the inhibition of prostaglandin synthesis by competitive blocking of the enzyme cyclooxygenase (COX).

Protocol
Cell Experiment
Cell lines Primary human osteoblasts cell lines
Preparation method Exposing human osteoblasts cells to Ketorolac for 24 hours.Assessing thymidine incorporation by the TopCount Microplate Scintillation and Luminescence Counters through adding [3H]-thymidine to cultures 4 hours prior to harvesting. Using propidium iodide in flow cytometer to determine cell cycle distribution , and using the Annexin V-FITC Apoptosis Detection Kit to detect cell apoptosis or necrosis.
Concentrations Dissolved in DMSO, final concentration ~0.1 mM
Incubation time 24 hours
Animal Experiment
Animal models Male Wistar rats
Formulation Dissolved in DMSO and diluted in saline.
Dosages 0.3-30 mg/kg
Administration Take orally
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 376.4
Formula C19H24N2O6
CAS Number 74103-07-4
Purity >99%
Solubility DMSO 70 mg/mL
Storage at -20°C
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Tenoxicam

Tenoxicam is a good HO. radicals scavenger with an IC50 of 56.7 μM.

Suprofen

Suprofen is a dual COX-1/COX-2 inhibitor, used as a non-steroidal anti-inflammatory analgesic and antipyretic.

Salicylic acid

Salicylic acid is a beta hydroxy acid that occurs as a natural compound in plants which is an inhibitor of ethylene biosynthesis and cyclooxygenase activity.

Propyphenazone

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Ketorolac supplier, COX, inhibitors

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