Ketorolac (Toradol) is a non-selective COX inhibitor with IC50 of 31.5 µM and 60.5 µM for human recombinant COX-1 and COX-2, respectively. Ketorolac tromethamine is a non-steroidal anti-inflammatory agent (NSAID) in the family of heterocyclic acetic acid derivative, often used as an analgesic, antipyretic (fever reducer), and anti-inflammatory. Ketorolac tromethamine (Toradol) acts by inhibiting the bodily synthesis of prostaglandins. The primary mechanism of action responsible for ketorolac’s anti-inflammatory, antipyretic and analgesic effects is the inhibition of prostaglandin synthesis by competitive blocking of the enzyme cyclooxygenase (COX).
MedComm (2020). 2023 Sep 20;4(5):e363.
Arachidonic acid metabolism in health and disease
Ketorolac purchased from AbMole
Cell Experiment | |
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Cell lines | Primary human osteoblasts cell lines |
Preparation method | Exposing human osteoblasts cells to Ketorolac for 24 hours.Assessing thymidine incorporation by the TopCount Microplate Scintillation and Luminescence Counters through adding [3H]-thymidine to cultures 4 hours prior to harvesting. Using propidium iodide in flow cytometer to determine cell cycle distribution , and using the Annexin V-FITC Apoptosis Detection Kit to detect cell apoptosis or necrosis. |
Concentrations | Dissolved in DMSO, final concentration ~0.1 mM |
Incubation time | 24 hours |
Animal Experiment | |
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Animal models | Male Wistar rats |
Formulation | Dissolved in DMSO and diluted in saline. |
Dosages | 0.3-30 mg/kg |
Administration | Take orally |
Molecular Weight | 376.4 |
Formula | C19H24N2O6 |
CAS Number | 74103-07-4 |
Solubility (25°C) | DMSO 70 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Ewan D McNicol, et al. Ketorolac for postoperative pain in children
[3] Benjamin Maslin, et al. Safety Considerations in the Use of Ketorolac for Postoperative Pain
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