Kaempferol 3-O-β-D-glucuronide is a conjugated kaempferol metabolite, which has anti-inflammatory effect. Kaempferol 3-O-β-D-glucuronide significantly inhibits various pro-inflammatory mediators like IL-1β, NO, PGE2, and LTB4.
| Cell Experiment | |
|---|---|
| Cell lines | RAW 264.7 cells |
| Preparation method | RAW 264.7 cells (2×105cells/well) were pretreated with K3G (5 and 10 μM) for 1 h and stimulated with LPS (1 μg/mL) for 24 h. Cells were then harvested. Cell pellet was solubilised in RIPA buffer (Sigma; R-0278) over ice for 40 min. The lysate was centrifuged at 14,000 g for 15 min at 4 °C. Supernatant was collected. Protein estimation was done by Bradford's method with BSA standard. Western blotting was performed according to the reported protocol. Detection of specific proteins was done by Immobilon Western (Chemiluminescent HRP substrate, Millipore) mediated chemiluminescence by ChemiDoc XRS+(Bio Rad). Densitometry of specific bands was done using Image Lab Software version 3 (Bio Rad). The effect on phosphorylation of NF-kB p65 was studied in a dose dependent manner in comparison to dexamethasone (0.5 μM). |
| Concentrations | 5 and 10 μM |
| Incubation time | 1 h |
| Animal Experiment | |
|---|---|
| Animal models | Female Balb/C mice 10–12 weeks old and weight is 20–25 g |
| Formulation | dissolved in 100% DMSO and the working concentration of DMSO in animals is below 1% |
| Dosages | 1 mg/kg, 2 mg/kg and 5 mg/kg |
| Administration | drugged for 1 h |
| Molecular Weight | 462.36 |
| Formula | C21H18O12 |
| CAS Number | 22688-78-4 |
| Solubility (25°C) | DMSO |
| Storage | 2-8°C, protect from light, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related IL Receptor/Related Products |
|---|
| 13-Methylberberine chloride
13-Methylberberine chloride is a berberine analogue, has anti-adipogenic and antitumor activities. 13-Methylberberine chloride increases production of IL-12 and inhibits the expression of iNOS at posttranscriptional level in macrophages activated with LPS. |
| HB0017
HB0017 is a monoclonal antibody that targets interleukin-17 (IL-17) and selectively binds IL-17A, thereby preventing IL-1717A from binding to its receptor. |
| Rademikibart
Rademikibart is a human IgG4ҡ monoclonal antibody that targets IL-4 Rα to block inflammatory signaling induced by IL-4 and IL-13, two important cytokines that drive allergic inflammation, and binds to the human IL-4Rα epitope with a KD of 20.7 pM. Rademikibart does not bind to IL-4Rα from other species. Rademikibart does not bind to IL-4Rα of other species.Rademikibart inhibits IL-4 and IL-13-mediated STAT6 signaling, TF-1 cell proliferation, and TARC production in PBMC, and can be used in studies related to moderate-to-severe Th2 inflammatory diseases. |
| Stapokibart
Stapokibart is a humanized monoclonal antibody targeting IL-4Rα that blocks the interaction of cytokines IL-4 and IL-13 with their co-receptor subunit, IL-4Rα. Stapokibart is able to interact with IL-4Rα in humans, cynomolgus monkeys, and rats, and can be used in atopic dermatitis research. |
| Erepdekinra
Erepdekinra is an interleukin-17A (IL-17A) receptor antagonist. |
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