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IWP-2

Cat. No. M2237
IWP-2 Structure
Synonym:

Inhibitor of Wnt Production-2

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mg USD 85 In stock
50mg USD 285 In stock
100mg USD 430 In stock
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Quality Control
Biological Activity

IWP-2 is an inhibitor of Wnt production. IWP-2 inactivates Porcupine, a membrane-bound O-acyltransferase responsible for palmitoylating Wnt proteins, which is essential for their signaling ability and secretion. IWP-2 selectively inhibits palmitoylation of Wnt, and blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. IWP-2 is useful in both suppressing embryonic stem cell self-renewal and decreasing cancer cell proliferation, migration and invasion.

Customer Product Validations & Biological Datas
Source Front Immunol (2018). Figure 6. IWP-2
Method injected intravenously
Cell Lines C57BL/6 mice
Concentrations 20 mg/kg
Incubation Time 4.5 h
Results This view is supported by reduced percentages of IFN-γ+ liver CD3+NK1.1+ cells in mice treated with IWP-2, a small molecule inhibitor of the acyltransferase Porcupine, which plays a central role in Wnt secretion
Protocol
Cell Experiment
Cell lines MKN28 cell line
Preparation method Cell proliferation assay CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay kit (Promega Corporation, Madison, WI, USA) was used. Transwell migration and invasion assay Haptotaxis chambers (8-μm pore size, Corning Costar, Cambridge, MA, USA) without or with 5 mg/ml matrigel (Sigma Aldrich) were used for migration and invasion assays, respectively. The experiments were repeated three times independently.
Concentrations 0,5,10,20,50µM
Incubation time 5 days
Animal Experiment
Animal models six month-old zebrafish model
Formulation 0.1% DMSO
Dosages 10 μM
Administration in aquarium water supplemented with 10 μM IWR-1 or in 0.1% DMSO as a control
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 466.6
Formula C22H18N4O2S3
CAS Number 686770-61-6
Purity >99%
Solubility DMSO
Storage at -20°C
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: IWP-2, Inhibitor of Wnt Production-2 supplier, Wnt/beta-catenin, inhibitors

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